Shared Facilities Research

Functional Genomics Core Lab

Scientific Director: Willard Freeman, PhD

The Functional Genomics Core Lab provides services for measuring changes in gene expression, such as RNA quality assessment, microarray analyses for measuring changes in global mRNA expression, custom microarray production services, and Quantitative Real-Time PCR analyses for measuring changes in specific mRNA levels.  SNP analysis services are also provided. The Department of Public Health Sciences provides supplementary microarray analysis services for biostatistical analysis.

Services Offered:

·         RNA Quality Assessment

·         DNA Microarray Services (dye-attachment, hybridization, and analysis)

·         Quantitative Real-Time PCR analyses

·         TaqMan SNP analyses

·         Custom DNA microarray production

·         Protein Array production

·         Laser Capture Microdissection

Major Equipment:

·         Agilent Bioanalyzer 2100

·         Axxon Genepix 4000B, Packard ScanArray4000XL microarray scanners

·         Apogent Discoveries MicroGrid arrayer

·         MWG RoboSeq 4204S Liquid Handling Robot

·         Analysis with GeneSpring GX 7.3 software (Silicon Genetics)

·         SpectraMax 2 Fluorescence and absorbance plate reader