Shared Facilities Research

Animal Research Services: Transgenic Services

Scientific Director: Sarah Bronson, PhD   

The purpose of the Transgenic Core Lab is to centralize development of transgenic and targeted gene disruption mouse models for use in biomedical research, since experience with similar facilities at other institutions indicated that the efficiency and success rates of transgenic experiments are greatly improved in a core facility where the procedures are conducted routinely. This centralization of the basic microinjection and stem cell technologies in a common core lab also avoids unnecessary and expensive duplication of efforts.  To allow this centralization, an 8 x159 sq. ft addition to the Animal Research Farm, funded in part by a C06 grant, was built to house transgenic animals. 

The new wing was designed to operate as a barrier utilizing structural factors and procedures to prevent the introduction of infectious diseases into the animals housed there.  Investigators have access to the Transgenic Animal Facility on a fee-for-service basis.  Several investigators at the College of Medicine are currently funded for work involving transgenic animals.  The College of Medicine provides funds for the purchase of equipment for microinjections, and for technical support.

Services Offered:

·         Production of Transgenic Mice

·         Production of Knockout Mice

·         Rederivation Services

Major Equipment:

·         Leica Labovert FS microscope with dual micromanipulators

·         Sutter Flaming/Brown Micropipette Puller P-87

·         Narashige microforge MF-9

·         Olympus SZH10 dissecting stereoscope

·         Eppendorf Transinjector 5246 system