Morphologic and Molecular Pathology Core Research Lab (MMPCRL)
Resources and Technical
Histology and Histochemistry
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Fixation and processing
of human or animal tissues, cell culture flasks or pellets
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Paraffin embedding and
sectioning of tissues
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Hematoxylin and eosin
(H&E) staining of sections
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Embedding, cryostat
sectioning and staining of fresh, frozen tissues
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Customized histochemical
stains
 
Immunohistochemistry
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Immunohistochemical
staining of human or animal tissues, cell culture flasks or cell culture
pellets
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Mouse-on-mouse/rat
immunohistochemistry
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Chromogenic and
fluorescent detection methods
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Multiple antigen
labeling techniques
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Automated
immunohistochemical staining performed on a DakoCytomation Autostainer Plus,
for large volume studies
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Professional assistance
in the identification of the optimal commercial or research sources for
antibodies and identification of best control tissues
 
In situ Hybridization
Tissue Microarray
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Tissue microarrays can
be created with any combination of human tissue, animal tissues or cell
culture pellets
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Arrays are constructed
using the Beecher MTA-I
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Tissue core sizes can
range from 0.6 mm to 2.0 mm, providing from 40 to over 200 sample cores in
one paraffin block
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Microarray slides can be
stained using a variety of histochemical or immunohistochemical reactions
DNA
Extraction
Laser Capture
Microdissection
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Laser capture microdissection is now
available using the Zeiss PALM Microbeam.
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Capable of isolating individual cell and
tissue types from both frozen and paraffin sections.
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Ideal method of insuring the purity of
starting material for downstream proteomic and genomic applications.
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Lab is capable of
operating on an RNase free level to insure high quality samples for genomic
studies.
Gene expression Array (under negotiation, probable availability, November, 2008)
Tumor, Tissue, Serum and
Urine Bank
(located in the MMPCRL, C7718B)
See:
http://www.hmc.psu.edu/cancer/tissue_bank/tissue_main.htm for more detail regarding
available specimens, application forms, etc.
As of September, 2008:
Tissues available ~8000
tumor ~2500
plasma, buffy coat, RBC ~20,000
buccal smears ~1300
Tissues distributed ~ 1500
to 34 researcher
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