|
|
|
|
Faculty Biosketch
|
Milton S. Hershey
Medical Center
Penn State College of Medicine
P.O. Box 850,
500 University Drive
Hershey, PA 17033-2390 |
|
Willard M. Freeman, PhD
Department of Pharmacology
|
 |
Office Information
Phone: 717-531-4037
Mail Code: H078
|
|
Education |
B.A., Wake
Forest University, 1994
Ph.D., Wake Forest University School of Medicine, 2001
Post Doc, Vollum Institute, Oregon Health Sciences
University, 2001-2003
Yerkes Primate Center, Emory Universtiy, 2003-2004 |
| Primary
Area of Interest |
|
The focus of the laboratory is the systems biology
examination of psychiatric disorders. |
| Research
Focus |
| The primary goal of my research
is to better understand the molecular/biochemical basis of
behavior. This research goal requires combining advanced
molecular biological and biochemical techniques with
behaviorally meaningful, whole animal designs. We approached
this question from a systems biology perspective using
discovery tools such as quantitative proteomics, microarrays,
and bioinformatics to examine gene and protein changes in
different brain regions.
Specifically, we are interested in the effects of drug
abuse (cocaine and amphetamine), alcohol abuse, and
schizophrenia on gene expression within the brain. |
| References |
|
- Freeman WM, Gooch RS, Worst TJ, Walker SJ, Xu AS, Green H, Pierre PJ,
Grant KA, Vrana KE. ApoAII is an elevated biomarker of chronic non-human
primate ethanol self-administration. Alcohol & Alcoholism 2006, In Press.
- Freeman WM, Lull ME, Guilford M, Vrana KE. Abundant protein depletion
from non-human primate serum for biomarker studies. Proteomics 2006; In
Press.
- Penn State Retina Research Group. Diabetic Retinopathy: Seeing beyond
glucose-induced microvascular disease. Diabetes 2006; In Press.
- Freeman WM, Brebner K, Amara SG, Reed MS, Pohl J, Phillips AG. Distinct
proteomic profiles of amphetamine self-administration transitional states.
The Pharmacogenomics Journal 2005; 5:203-14.
- Worst TJ, Tan JC, Robertson DJ, Freeman WM, Hyytia P, Kiianmaa K, Vrana
KE. Transcriptome analysis of frontal cortex in alcohol-accepting and
non-accepting rats. J. Neuroscience Research 2005; 80:529-538.
- Stedrick DL, Stokes AH, Worst TJ, Freeman WM, Johnson EA, Lash LH,
Aschner M, Vrana KE. Manganese-induced cytotoxicity in
catecholamine-producing cells. NeuroToxicology 2004; 25:543-53.
- Walker SJ, Worst TJ, Freeman WM, Vrana KE. Functional genomic analysis
in pain research using hybridization arrays. Methods in Molecular Medicine.
2004; 99:239-54.
- Tang W, Wesley M, Freeman WM, Liang B, Hemby SE. Alterations in
ionotropic glutamate receptor subunits during binge cocaine
self-administration and withdrawal in rats. J. Neurochemistry 2004; 89:
1021-1033.
- Freeman WM, Hemby SE. Proteomics for protein expression profiling in
neuroscience. Neurochemical Research 2004; 29:1065-1081.
- Vrana KE, Freeman WM, Aschner M. Use of microarray technologies in
toxicology research. NeuroToxicology 2003; 24:321-332.
- Stokes AH, Freeman WM, Mitchell SG, Burnette TA, Hellmann GM, Vrana KE.
Induction of GADD45 and GADD153 in neuroblastoma cells by dopamine induced
neurotoxicity. NeuroToxicology 2002; 23:675-684.
- Freeman WM, Brebner K, Patel KM, Lynch WJ, Roberts DCS, Vrana KE.
Repeated cocaine self-administration causes multiple changes in rat frontal
cortex gene expression. Neurochemical Research 2002; 27:1181-1192.
- Freeman WM, Brebner K, Lynch WJ, Robertson DJ, Patel KM, Roberts DCS,
Vrana KE. Changes in rat frontal cortex gene expression following chronic
cocaine. Molecular Brain Research 2002; 104:11-20.
- Freeman WM, Vacca SE, Dougherty K, Vrana KE. An interactive database of
cocaine-responsive neuronal gene expression. The Scientific World 2002;
2:701-706.
- Freeman WM, Brebner K, Lynch WJ, Robertson DJ, Roberts DCS, Vrana KE.
Cocaine-responsive gene expression changes in rat hippocampus. Neuroscience
2001; 108:371-380.
- Freeman WM, Nader MA, Nader SH, Robertson DJ, Gioia L, Mitchell SM,
Daunais JB, Porrino LJ, Friedman DP, Vrana, KE. Chronic cocaine-mediated
changes in non-human primate nucleus accumbens gene expression. Journal of
Neurochemistry 2001; 77:542-849.
- Freeman WM, Robertson DJ, Vrana KE. Fundamentals of DNA hybridization
arrays for gene expression analysis. BioTechniques 2000; 29:1042-1055.
- Freeman WM, Yohrling GJ, Gioia L, Daunais J, Porrino L, Vrana KE. A
novel cocaine analog 2ƒÒ-propanoyl-3ƒÒ-(4-tolyl)-tropane reduces tyrosine
hydroxylase gene expression in the mesolimbic dopamine pathway. Drug and
Alcohol Dependence 2000; 61:15-21.
- Freeman WM, Walker SJ, Vrana KE. Quantitative RT-PCR: Pitfalls and
potential. BioTechniques 1999; 26:112-125.
Back
|
|
|
