Proteins & Mass Spectrometry
Gel
Imaging and Analysis
Phosphorimager Gel/Blot
Analysis (1D and 2D)
For direct quantitation of
radioactive bands in dried gels
or blots, a
BioRad Molecular Imager FX Pro
Plus PhosphorImager is
available, with a
linear range of detected density
of 5 orders of magnitude.
Price is $2 per scan.
To schedule
service, please contact Anne
Stanley (x6087, email
astanley@psu.edu) or Suja
Maddukuri (x6087, email
srm25@psu.edu).
Fluorimager Gel/Blot Analysis
(1D and 2D)
The FX Pro
Plus also has a 3-laser
Fluorimager (488, 532, & 635
nm lasers)
which enables band and spot
detection from a large variety
of fluors. After
image acquisition, Quantity One
1D or PDQuest 2D analysis
software can be
used to perform calculations of
band densities, background
corrections, etc. The
PDQuest software can also
compare spot densities on
different gel images to select
protein spots whose levels
change between different
experimental conditions, and a
spot cut list can be exported to
our LEAP/BioMachines 2DiDx gel
spot cutter.
The
imaging charge is $2 per scan, and
the instrument is available on a
sign-up
basis to individual trained
users.
To schedule
service, please contact Anne
Stanley (x6087, email
astanley@psu.edu) or Suja
Maddukuri (x6087, email
srm25@psu.edu).
Gel/Film Densitometry Analysis
(1D and 2D)
For
quantitation of exposed film
(radioactive or chemiluminescent
detection), a BioRad GS800
Calibrated Densitometer is
available to digitize
film images. The densitometric
image obtained can then be
analyzed using
Quantity One software to analyze
1D gels or slot/dot blot images,
or PDQuest
software to analyze
two-dimensional gel images. The
rate for Densitometry is
$5/hr, and the instrument is
available to trained users on a
sign-up basis.
To schedule
service, please contact Anne
Stanley (x6087, email
astanley@psu.edu) or Suja
Maddukuri (x6087, email
srm25@psu.edu).
DIGE Gel
Analysis (2D)
For
quantitation of differences in
protein amount between different
samples, equal amounts of each
sample can be labeled with
different fluors, usually a
combination of Cy2, Cy3, and
Cy5, then mixed together before
2D gel separation. Fluorescent
imaging and quantitation of the
resulting 2D gels can be done
with our Typhoon Imager, and
spot-cut lists can be exported
for automated gel spot excision
using our Ettan Spot Picker.
To schedule
service, please contact Dr. BIll
Freeman (x4037, email
wfreeman@psu.edu )
Page
maintained by
Bruce A. Stanley
Last modified
May 29, 2008 09:23 PM
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