Proteins and Mass Spectrometry

Instrumentation and Services available

Major Equipment:

• ABI/MDS Sciex 4800 MALDI TOF TOF mass spec

• ABI 4700 Proteomics Analyzer MALDI TOF-TOF mass spec

• MDS Sciex 4000 QTrap hybrid ion trap mass spec

• ABI Voyager DE-PRO Reflectron MALDI TOF mass spec

• Bioinformatics: ProteinPilot and Phenyx advanced protein-MS ID software; GPS Explorer and Mascot software, Analyst software

• HPLC and Gel Separation Systems:

  • Tempo LC-MALDI nanoflow separation & MALDI spotting system

  • Eksigent 2D nanoLC liquid chromatography system

  •  LC Packings/Dionex ProBot MALDI spotting robot

  • Agilent 1100, Shimadzu LC-AS10, Waters 600E HPLC systems

  • Beckman-Coulter PF 2D Whole Protein separation system

  • 12-gel casting and running apparatus for 2D gels (Ettan IPGPhor II and Dalt 12)

• Gel Imaging and analysis

  • BioRad FX Pro Plus phosphorimager, fluorimager (3 laser), and Densitometer with QuantityOne and PDQuest software

  • GE/Amersham Typhoon DIGE gel reader with DeCyder software

• Spot-cutting and robotics

  • LEAP Technologies/BioMachines 2DiDx Spot-cutting/digestion/spotting robot

  • GE/Amersham Ettan Spot Picker

  • Tecan Robotics platform

• Shimadzu CHiP printer

• Wyatt DAWN HELEOS light-scattering detection system for protein sizing

Services Offered:

• Mass Spectrometry analyses and identification of proteins, peptides, oligonucleotides, carbohydrates, small molecules

• 2D LC separations of complex protein and/or peptide mixtures for Proteomicss

• Protein Expression analysis using ICAT & iTRAQ methodology

• Protein Expression analysis using 2D Gel-image analysis

• Automated spot-cutting, proteolytic digestion, and sample deposition from gels

• Shared equipment for analyzing whole protein sizes and multimeric state

Page maintained by Bruce A. Stanley
Last modified January 24, 2008 11:37 AM          This page has been viewed times since July 2007