The Functional Genomics  Microarray Facility provides the following services and Equipment:

Consultations on Experimental Design & Data Validation
RNA, DNA, Protein Quality Control
Arrays, Hybridization & Image Acquisition from any Commercial Spotted Array Platform
Data Analysis
Laser Capture Microdissection
Updated** Quantitative Real-Time PCR Analyses ** UPDATED  6/7/07
 

Important Instrumentation Purchases and services have been made possible through Tobacco Settlement Funds (TSF), Juvenile Diabetes Research Foundation funds (JDRF), and through the Penn State Cancer Institute (PSCI) contract with the Department of the Navy.

You will need an HMC ePASS userID and password to log on to any of our computers for analysis or data transfer purposes. For information on how to obtain an ePASS password, click here.

The Director of the Functional Genomics Core Facility is
Dr. Bill Freeman, Department of Pharmacology wfreeman@psu.edu
(717) 531-4037

The Functional Genomics Core is now located in the Hershey Center for Applied Research

Room 3200 

(717) 531-5823

RNA/DNA  QC/QA

• RNA QC Samples to be submitted by filling out THIS QC Sample Submission FORM and saving into the Functional Genomics Incoming QC Submission Folder on the network drive by using Print as pdf or if you have Adobe pro, use the File .. Save As ( Note:  The computer in C1734B has Adobe Pro installed on it ). 

Samples are to be put into marked box in C1734.

• Map your network to this address

\\hersheymed\files\Research\CoreFacility\Results\Functional Genomics Incoming\Incoming QC submission

• If you can not Print as a pdf, then use this Excel Form and Save As _ into the folder above.

• Note:  Use your Name or other Unique description and please make sure the tubes are labelled similarly with something descriptive, as well.

A Calendar has been set up to allow you to sign up for our Genomics and Proteomics Instruments and Services.  Please go to http://genomics.calendarhost.com

Functional Genomics personnel will also mark time off on this calendar so you can plan accordingly if you require assistance.

The daily operation of the

Microarray and qRT-PCR facility

(HCAR 3200) is the responsibility of:

Rob Brucklacher

&

Georgina Bixler

Please communicate by email whenever possible (Click their names above to send email).  This will allow experiments to be done in a timely fashion and you will be responded to as soon as possible.

Click HERE to download our brochure (PDF) with Current (September 2005) Prices and Services

(this will be updated as soon as possible)

qPCR

• Current charge per run on for ABI7300 is $25. ABI7900 is $50/run.
• The Core has qPCR reagents ( Plates, Master Mixes, Endogenous Controls )which are charged based on amount used.

Microarrays

• For our current microarray platform, we utilize Illumina arrays.
• Our previous microarray platform, Codelink, is again available from an alternate supplier, as well.

Please contact Rob Brucklacher to set up Microarray discussion and suggestions.

Laser Capture Microdissection

• A Zeiss P.A.L.M. LCM is maintained by the Core as a shared instrument.  We do not offer sectioning and LCM as a service.   We are available to schedule training.

The Department of Public Health Sciences is also available as experts in bioinformatics related to microarray analysis - click link below:

(Biostatistics & Bioinformatics - Microarrays and Statistical Genomics Consulting and Data Analysis)

When using data or tools generated here in publications or grants, please cite "Rob Brucklacher and/or Bill Freeman and/or Georgina Bixler of the Functional Genomics Core Facility of the Section of Research Resources, Penn State College of Medicine" as appropriate.

Please also let us know about publications or grant applications that make use of the services of this facility - this is important for continued availability of these services!