Gene
Expression Analysis
RealTime PCR for Quantitating
mRNA and DNA
Companies producing probes that can be
used for Quantitative Realtime PCR:
Additional Primer Programs on the WEB
for Realtime PCR primer design
(This information included for informational purposes
only, the Applied Biosystems program (Primer Express)
which we have is probably your
best bet for primer design for
the Appled
Biosystems 7900HT)
Some references:
Holland, P.M., Abramson, R.D., Watson, R. and Gelfand, D.H.
(1991) Detection of specific polymerase chain reaction
product by utilizing the 5' to 3' exonuclease activity of
Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci.
USA 88, 7276-7280.
Tyagi, S. and Kramer, F.R. (1996) Molecular beacons:
probes that fluoresce upon hybridization. Nat. Biotechnol.
16, 49-53.
Wittwer, C.T., Herrmann, M.G., Moss, A.A. and
Rasmussen, R.P. (1997) Contiuous fluorescence monitoring
of rapid cycle DNA amplification. Biotechniques 22,
130-138.
Rasmussen, R., Morrison, T., Herrmann, M. and Wittwer,
C. (1998) Quantitative PCR by continuous fluorescence
monitoring of a double strand DNA specific binding dye.
Biochemica 2, 8-11.
Nitsche, A., Steuer, N., Schmidt, C.A., Landt, O. and
Siegert, W. (1999) Different real-time PCR formats
compared for the quantitative detection of human
cytomegalovirus DNA. Clin. Chem. 45, 1932-1937.
Winer, J., Jung, C.K.S., Shackel, I. and Williams, M.
(1999) Development and validation of real-time
quantitative reverse transcriptase-polymerase chain
reaction for monitoring gene expression in cardiac
myocytes in vitro. Anal. Biochem. 270, 41-49.
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