Gene
Expression Analysis
Overview
Functional Genomics Core
Facility offers:
-
Consultations on
Experimental Design & Data
Validation
- RNA,
DNA, Protein Quality Control
-
Gene
expression Microarray Analysis
( mRNA and miRNA )
-
SNP/CNV
Microarray Analysis
- Data
Analysis
-
Quantitative Real-time PCR
Analysis
( RNA, DNA, SNP )
|
 |
The Functional Genomics Core
Facility is located in the
Hershey Center for Applied
Research, Room 3200.
A
calendar has been set up for
scheduling time on instruments.
Functional Genomics personnel
will also list up-coming time off
on this calendar so
you can plan accordingly if you
require assistance.
http://genomics.calendarhost.com/
Important
Instrumentation Purchases and Services have been made possible
through Tobacco Settlement Funds (TSF), Juvenile Diabetes
Research Foundation funds (JDRF), and through the Penn State
Cancer Institute (PSCI) contract with the Department of the
Navy.
|
When using data or
tools generated here
in publications or
grants, please cite
the Core Laboratory
personnel who worked
on the project
and the Functional Genomics Core
Facility of the Section
of Research Resources,
Penn State College of
Medicine" as
appropriate.
Please also let us know
about publications or
grant applications that
make use of the services
of this facility - this
is important for
continued availability
of these services! |
Equipment
ABI 7900HT sequence
detection system with
autoloader - 384 well
ABI 7300 sequence detection
system - 96 well
Agilent Bioanalyzer 2100
Illumina Bead Station
Illumina Bead
Xpress
Molecular Devices SpectraMax M2
MicroPlate Reader
NanoDrop ND-1000
Axon
Industries GenePix 4000B scanner
Zeiss P.A.L.M Laser
Capture -- Department of Pathology
( Contact:
Trey Bruggeman )
Recent
Publications
2008
1.
Fort PE, Freeman WM, Kimball SR,
Singh RSJ, Gardner TW. The
retinal proteome in
experimental diabetic
retinopathy: Upregulation of
Beta and Gamma-crystallins
and reversal by insulin.
Mol. Cell. Proteomics;
Dec 1. [Epub ahead of
print].
2.
Brucklacher RM, Patel
KM, Bixler GV, VanGuilder
HD, Antonetti
DA, Lin C-M, LaNoue
KM, Barber AJ, Gardner TW,
Bronson SK, Freeman
WM. Whole genome assessment of
the retinal response to diabetes
reveals a progressive
neurovascular inflammatory
response. BMC Medical
Genomics 2008; 1:26. [PMID:
18554398]
3.
VanGuilder HD, Ellis RW,
Brucklacher RM, Freeman WM,
Barber AJ. Streptozotocin-diabetes
decreases synaptic protein
expression in rat retina.
Eur. J. Neuroscience 2008;
28:1-11.
4.
Barthéléry M, Salli U,
Freeman WM, Vrana KE. 2-DIGE
identification of differentially
expressed hNRNPs and
transcription factors during
neural differentiation of human
ES cells, Proteomics:
Clinical Applications; In
press
5.
Mitchell RM, Freeman WM,
Randazzo WT, Stephens HE,
Simmons Z, Connor JR. A CSF
biomarker panel for
identification of patients with
amyotrophic lateral sclerosis.
Neurology 2008; Epub
ahead of print Nov 5.
6.
Jaishankar A, Barthéléry
M, Freeman WM, Salli U, Vrana KE.
Human Embryonic and Mesenchymal
Stem Cells Express Different
Nuclear Proteomes. Stem Cells
Develop. 2008; Sep 26. Epub
ahead of print
7.
Prokopczyk B, Sinha I, Trushin
N, Freeman WM, El-Bayoumy K.
Gene expression profiles in HPV-immortalized
human cervical cells treated
with the nicotine-derived
carcinogen
4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone.
Chemico-Biological
Interactions; 2008 Nov 6. [Epub
ahead of print].
8.
Freeman WM, Van Guilder HD,
Vrana KE. 25 years of
quantitative PCR for gene
expression analysis.
BioTechniques 2008; 44:
619-626.
9.
Lull ME, Freeman WM,
Vrana KE, Mash DC. Correlating
human and animal studies of
cocaine abuse and gene
expression. Annal. NY Acad.
Sci.; 2008
1141:58-75
10.
Kuntz KL, Patel KM, Grigson PS,
Freeman WM, Vrana KE.
Withdrawal persistent and
contingency-specific gene
expression changes with heroin
self-administration.
Pharm.
Biochem. & Behavior
2008; 90:349-356.
11.
Freeman WM, Patel KM, Lull ME,
Erwin MS, Bruchlacker RM, Morgan
D, Roberts DCS, Vrana KE.
Persistent alterations in
mesolimbic gene expression with
abstinence from cocaine
self-administration.
Neuropsychopharmacology
2008; 33:1807-1817.
12.
Bowyer JF, Pogge AR,
Delongchamp RR, O’Callaghan JP,
Patel KM, Vrana KE, Freeman WM.
A threshold
neurotoxic amphetamine
exposure inhibits parietal
cortex expression of synaptic
plasticity-related genes.
Neuroscience 2007;
144(1):66-76.
|
