Gene
Expression Analysis
Overview
Functional Genomics Core
Facility offers:
-
Consultations on
Experimental Design & Data
Validation
- RNA,
DNA, Protein Quality Control
-
MicroArray and Analysis
- Data
Analysis
-
Quantitative Real-time PCR
Analysis
( RNA, DNA, SNP )
-
Access to
Laser Capture
Microdissection
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The Functional Genomics Core
Facility is now located in the
Hershey Center for Applied
Research, Room 3200.
A
calendar has been set up for
scheduling time on instruments.
Functional Genomics personnel
will also list up-coming time off
on this calendar so
you can plan accordingly if you
require assistance.
http://genomics.calendarhost.com/
Important
Instrumentation Purchases and Services have been made possible
through Tobacco Settlement Funds (TSF), Juvenile Diabetes
Research Foundation funds (JDRF), and through the Penn State
Cancer Institute (PSCI) contract with the Department of the
Navy.
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When using data or
tools generated here
in publications or
grants, please cite
the Core Laboratory
personnel who worked
on the project
and the Functional Genomics Core
Facility of the Section
of Research Resources,
Penn State College of
Medicine" as
appropriate.
Please also let us know
about publications or
grant applications that
make use of the services
of this facility - this
is important for
continued availability
of these services! |
Equipment
ABI 7900HT
sequence detection system with
autoloader - 384 well
ABI 7300
sequence detection system - 96
well
Agilent
Bioanalyzer 2100
Molecular
Devices SpectraMax M2 MicroPlate Reader
NanoDrop
ND-1000
Zeiss
P.A.L.M Laser Capture
Microdissection
Publications & Posters
Papers:
Herzog CR, Blake DC,
Sun Y-W, Brucklacher RM,
Freeman WM. Forkhead box O3a
is a candidate
tumor suppressor gene deleted in lung adenocarcinomas. Cancer
Research, Submitted.
Ducker CE, Stettler EM,
Brucklacher RM, Freeman WM,
Charles D. Smith. HIP14 directly
affects Ras transformation.
Oncogene, Submitted.
VanGuilder HD, Ellis
RW, Brucklacher RM, Freeman
WM, Barber AJ.
Streptozotocin-diabetes
decreases synaptic protein
expression in rat retina, Exp.
Eye Res., In Press.
Gregg Ward, Steven Franklin, Dan
Krissinger, Kruti Patel, Kent
Vrana, Allyn Howlett:
Glucocorticoids plus opioids
upregulate genes that influence
synaptic plasticity. In review.
Bowyer JF, Pogge AR,
Delongchamp RR, O’Callaghan JP,
Patel KM, Vrana KE, Freeman
WM. A threshold neurotoxic
amphetamine exposure inhibits
synaptic plasticity gene
expression in the parietal
cortex. Neuroscience, In Press.
Reese BE, Krissinger D, Yun JK,
Billingsley ML. Elucidation of
stannin function using
microarray analysis:
implications for cell cycle
control.
Gene Expr.
2006;13(1):41-52.
Antonetti, DA, Barber AJ,
Bronson SK, Freeman WM,
Gardner TW, Jefferson LS, Kester
M, Kimball SR, Krady JK, LaNoue
KF, Nordbury CC, Quinn PG,
Sandirasegarane, Simpson IA.
Diabetic Retinopathy: Seeing
beyond glucose-induced
microvascular disease. Diabetes
2006; 55:2401-2411.
Worst TJ, Tan JC,
Robertson DJ, Freeman WM,
Hyytia P, Kiianmaa K, Vrana KE.
Transcriptome analysis of
frontal cortex in
alcohol-accepting and
non-accepting rats. J.
Neuroscience Research 2005;
80:529-538.
Gregg Ward, Steven Franklin, Dan
Krissinger, Kruti Patel, Kent
Vrana, Allyn Howlett. Effect of
stress and drugs of abuse on
patterns of gene expression in
SHSY-5Y human neuroblastoma
cells. Abstract. Experimental
Biology Meeting (FASEB), 2005.
California, 2005.
Inability of L22 ribosomal
protein alteration to increase
macrolide MICs in the absence of
efflux mechanism in Haemophilus
influenzae HMC-S. July 8, 2004.
J. Antimicrob. Chemother. 2004
54: 393-400.
Abstracts
Freeman WM, Patel
KM, Brucklacher RM, Thomas L,
Morgan D, Roberts DCS, Vrana KE.
Persistent mesolimbic gene
expression changes with cocaine
abstinence. SFN 2006.
Sonntag WE, Bennett CD,
Erwin MS, Lull ME, Freeman WM.
Age-related changes in the
hippocampal proteome in
cognitively defined rodents. SFN
2006.
Lull ME, Erwin MS, Patel
KM, Thomas L, Morgan D, Roberts
DCS, Freeman WM, Vrana KE,
The proteomic profile of rat
mPFC is altered following
long-term abstinence from
cocaine self-administration. SFN
2006.
Morgan D,
Freeman WM, Patel KM,
Roberts DCS, Vrana KE.
Persistent changes in medial
prefrontal cortex mRNA levels
following binge cocaine
self-administration and varying
durations of abstinence. CPDD
2006.
VanGuilder
HD, Ellis RW, Freeman WM,
Barber AJ. Streptozotocin-diabetes
descreases synaptic protein
expression in rat retina, ARVO
2006.
Patrice E Fort, Kirk A Gilbert,
Courtney L Bradley, Ravi S J
Singh, Chad E N Reiter, Ali
Reiter, Dan Krissinger, Leonard
S Jefferson, Scot R Kimball and
Thomas W Gardner(2006)
. Normal Rat Retina
Exhibits A Higher Protein
Synthesis Rate Than Skeletal
Muscle.
Freeman
WM, Krissinger DJ,
Brucklacher RM, Kimball S
Transcriptional alterations in
Müller cells with elevated
glucose. ARVO 2006.
Freeman
WM, Wesley MJ, Vrana KE,
Hopkins W. Proteomic
characterization of the
non-human primate brain. HUPO
20005, Munich.
Jennifer E. Nyland, Willard M.
Freeman, Alistair J. Barber,
Daniel J. Krissinger, Rhona W.
Ellis, and the Penn State Retina
Research Group.
(2005).
Investigating Retinal
Neuropathy in Diabetes Using
Laser Capture Microscopy.
Susan Bell Nyland, Dan
Krissinger, Nancy Ruth Jarbadan,
John W. Wills, and Thomas P.
Loughran, Jr.
(2005).
Serorecognition of
Lentivirus Antigens in LGL
Leukemia
Patel KM, Freeman WM, Brebner,
Fasoulo WH, Morgan D, Roberts
DCS, Vrana KE. Quantitative
RT-PCR confirmation of
prefrontal cortex gene
expression changes across a timecourse of rat cocaine
self-administration. 2004,
Society for Neuroscience.
Lee, J.H., Krissinger, D. and
Clawson, G. (2004).
Utilization of trans-acting
hammerhead ribozymes
to
characterize catalytic
nucleic acids in vitro and in
stably transfected cells.
Galderisi, C., Krissinger, D.,
Bozdogan, B. and Applebaum,
P.(2004). Comparative
Analysis of Gene
Expression in Seven
Haemophilus influenzae Strains
and Their Macrolide Resistant
Derivatives.
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