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Core Research Facilities

Gene Expression Analysis

 

Overview

Functional Genomics Core Facility offers:

  • Consultations on Experimental Design & Data Validation
  • RNA, DNA, Protein Quality Control
  • MicroArray and Analysis
  • Data Analysis
  • Quantitative Real-time PCR Analysis ( RNA, DNA, SNP )
  • Access to Laser Capture Microdissection

 

The Functional Genomics Core Facility is now located in the Hershey Center for Applied Research, Room 3200. 

A calendar has been set up for scheduling time on instruments.  Functional Genomics personnel will also list up-coming time off on this calendar so  you can plan accordingly if you require assistance.  

http://genomics.calendarhost.com/

Important Instrumentation Purchases and Services have been made possible through Tobacco Settlement Funds (TSF), Juvenile Diabetes Research Foundation funds (JDRF), and through the Penn State Cancer Institute (PSCI) contract with the Department of the Navy.

 

When using data or tools generated here in publications or grants, please cite the Core Laboratory personnel who worked on the project and the Functional Genomics Core Facility of the Section of Research Resources, Penn State College of Medicine" as appropriate.

Please also let us know about publications or grant applications that make use of the services of this facility - this is important for continued availability of these services!

 

 

Equipment

ABI 7900HT sequence detection system with autoloader - 384 well

ABI 7300 sequence detection system - 96 well

Agilent Bioanalyzer 2100

Molecular Devices SpectraMax M2 MicroPlate Reader

NanoDrop ND-1000

Zeiss P.A.L.M Laser Capture Microdissection

 

 

Publications & Posters

Papers:

Herzog CR, Blake DC, Sun Y-W, Brucklacher RM, Freeman WM. Forkhead box O3a is a candidate tumor suppressor gene deleted in lung adenocarcinomas. Cancer Research, Submitted.

Ducker CE, Stettler EM, Brucklacher RM, Freeman WM, Charles D. Smith. HIP14 directly affects Ras transformation.  Oncogene, Submitted.

VanGuilder HD, Ellis RW, Brucklacher RM, Freeman WM, Barber AJ. Streptozotocin-diabetes decreases synaptic protein expression in rat retina, Exp. Eye Res., In Press.

Gregg Ward, Steven Franklin, Dan Krissinger, Kruti Patel, Kent Vrana, Allyn Howlett: Glucocorticoids plus opioids upregulate genes that influence synaptic plasticity. In review.

Bowyer JF, Pogge AR, Delongchamp RR, O’Callaghan JP, Patel KM, Vrana KE, Freeman WM.  A threshold neurotoxic amphetamine exposure inhibits synaptic plasticity gene expression in the parietal cortex.  Neuroscience, In Press.

Reese BE, Krissinger D, Yun JK, Billingsley ML. Elucidation of stannin function using microarray analysis: implications for cell cycle control.  Gene Expr. 2006;13(1):41-52.

Antonetti, DA, Barber AJ, Bronson SK, Freeman WM, Gardner TW, Jefferson LS, Kester M, Kimball SR, Krady JK, LaNoue KF, Nordbury CC, Quinn PG, Sandirasegarane, Simpson IA. Diabetic Retinopathy: Seeing beyond glucose-induced microvascular disease. Diabetes 2006; 55:2401-2411.

Worst TJ, Tan JC, Robertson DJ, Freeman WM, Hyytia P, Kiianmaa K, Vrana KE.  Transcriptome analysis of frontal cortex in alcohol-accepting and non-accepting rats.  J. Neuroscience Research 2005; 80:529-538.

Gregg Ward, Steven Franklin, Dan Krissinger, Kruti Patel, Kent Vrana, Allyn Howlett. Effect of stress and drugs of abuse on patterns of gene expression in SHSY-5Y human neuroblastoma cells. Abstract. Experimental Biology Meeting (FASEB), 2005. California, 2005.

Inability of L22 ribosomal protein alteration to increase macrolide MICs in the absence of efflux mechanism in Haemophilus influenzae HMC-S. July 8, 2004. J. Antimicrob. Chemother. 2004 54: 393-400.

Abstracts

Freeman WM, Patel KM, Brucklacher RM, Thomas L, Morgan D, Roberts DCS, Vrana KE. Persistent mesolimbic gene expression changes with cocaine abstinence.  SFN 2006.

Sonntag WE, Bennett CD, Erwin MS, Lull ME, Freeman WM. Age-related changes in the hippocampal proteome in cognitively defined rodents. SFN 2006.

Lull ME, Erwin MS, Patel KM, Thomas L, Morgan D, Roberts DCS, Freeman WM, Vrana KE, The proteomic profile of rat mPFC is altered following long-term abstinence from cocaine self-administration. SFN 2006.

Morgan D, Freeman WM, Patel KM, Roberts DCS, Vrana KE. Persistent changes in medial prefrontal cortex mRNA levels following binge cocaine self-administration and varying durations of abstinence. CPDD 2006.

VanGuilder HD, Ellis RW, Freeman WM, Barber AJ. Streptozotocin-diabetes descreases synaptic protein expression in rat retina, ARVO 2006.

Patrice E Fort, Kirk A Gilbert, Courtney L Bradley, Ravi S J Singh, Chad E N Reiter, Ali Reiter, Dan Krissinger, Leonard S Jefferson, Scot R Kimball and Thomas W Gardner(2006) . Normal Rat Retina Exhibits A Higher Protein Synthesis Rate Than Skeletal Muscle.

Freeman WM, Krissinger DJ, Brucklacher RM, Kimball S Transcriptional alterations in Müller cells with elevated glucose. ARVO 2006.

Freeman WM, Wesley MJ, Vrana KE, Hopkins W.  Proteomic characterization of the non-human primate brain. HUPO 20005, Munich.

Jennifer E. Nyland, Willard M. Freeman, Alistair J. Barber, Daniel J. Krissinger, Rhona W. Ellis, and the Penn State Retina Research Group.  (2005). Investigating Retinal Neuropathy in Diabetes Using Laser Capture Microscopy. 

Susan Bell Nyland, Dan Krissinger, Nancy Ruth Jarbadan, John W. Wills, and Thomas P. Loughran, Jr.   (2005).  Serorecognition of Lentivirus Antigens in LGL Leukemia

Patel KM, Freeman WM, Brebner, Fasoulo WH, Morgan D, Roberts DCS, Vrana KE. Quantitative RT-PCR confirmation of prefrontal cortex gene expression changes across a timecourse of rat cocaine self-administration. 2004, Society for Neuroscience.

Lee, J.H., Krissinger, D. and Clawson, G. (2004). Utilization of trans-acting hammerhead ribozymes          to characterize catalytic nucleic acids in vitro and in stably transfected cells.

Galderisi, C., Krissinger, D., Bozdogan, B. and Applebaum, P.(2004). Comparative Analysis of Gene           Expression in Seven Haemophilus influenzae Strains and Their Macrolide Resistant Derivatives.  

 

 

 

 

 

 

 

 

 
 
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