Transgenic Animals
Generation of
Transgenic Mice
The
Transgenic Core Facility at the
Penn State College of Medicine
(PSCOM) provides a centralized
facility for the production of
transgenic mice. DNA
microinjection produces random
insertion transgenic mice. The
Transgenic Core will perform all
the steps necessary to produce
transgenic mice, including
set-up, embryo harvesting, DNA
microinjection and transfer to
recipient mothers. The
investigator is responsible for
supplying the purified construct
(DNA) prepared using our
DNA Guidelines and
DNA Preparation Protocol.
We will complete one set of
injections (minimum of 150
viable embryos implanted into
recipient females, 20 candidate
pups, or 2 founder pups,
whichever criteria is met first)
and supply the investigator with
tail biopsies of the resulting
pups. The investigator is
responsible for genotyping
potential founder mice. Once
founder mice have been
identified, the Transgenic Core
will transfer the animals to the
investigator.
 The
standard mouse strains used for
microinjection are B6D2F1, FVB/N
and C57Bl/6J or the investigator
can provide the mice with no
guarantee. If the investigator
requires microinjections on
another background strain, this
can be arranged for an
additional cost. For additional
information please refer the
Transgenic Core Facility
Guidelines.
For Fee Schedule, see
Transgenic and Knockout Mouse
Generation Fees
Statistics
Blastocyst injections
1997-present
24 cell lines producing
chimeras
125 chimeras from 989 pups
born- 12.6%
success rate
At least
10 lines have been
reported to be germline
Transgenic injections
2005-present
20 injections
188 pups tested
29 positive founders- 15.4%
success rate
(Before requiring DNA
quality Check)
1997-2004
299 injections
2242 pups tested
224 positive founders-10.0%
success rate
The Transgenic Core Facility
has injected 83
constructs for 23
investigators,
generating 253 transgenic
founders, averaging 3
founders per construct.
Rederivations
2005-present
13 rederivations for 6
investigators
112 pups born-8.6 pups per
rederivation
56 positive pups-4.3
positive pups per
rederivation
Major Equipment
-
Leica
Labovert FS microscope with
dual micromanipulators
-
Sutter
Flaming/Brown Micropipette
Puller P-87
-
Narashige
microforge MF-9
-
Olympus SZH10
dissecting stereoscope
-
Eppendorf
Transinjector 5246
-
Laminar flow hood
-
VWR CO2 incubator Model
2310
Related
Guidelines & Protocols
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