Transgenic Animals
Generation of Knock-out Mice
The Transgenic Core Facility
at the at the
Penn State College of Medicine (PSCOM)
provides a centralized facility
for the production of knockout
mice.
Investigators must
receive approval from the
Director of the Core, Dr. Sarah
Bronson, for a project to be
initiated.
Cells submitted to the
Core for injection, must be
tested against murine virus
prior to injection. The Core
Facility will inject the
investigator’s cells into the
blastocysts collected from 10
superovulated C57BL/6 females
and will transfer them into
recipient females.
Requesting
Service
Complete and submit an
Injection Request
Form for Knock-out
Mouse Production.
The Core will contact you to
schedule injection and the
delivery of cells to the Core.
Every request will be taken on a
first come, first serve basis.
Investigators must submit
cells prepared for injection to
the Core on the morning
injections are scheduled.
Embryonic stem cells must be of
excellent quality in order to
achieve germline transmission of
mutations.
Investigators must
perform the breeding of chimeras
to obtain germline transmission.
Core personnel will be available
to answer any questions.
For more information, please see
Scheduling Services.
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