Peptide Synthesis

After submitting an order, you should see a Confirmation Page . If you DO NOT, then email your order to astanley@psu.edu.

Even if you see the confirmation page, if you do not get an email confirming your order within one day, check directly with the lab. (Anne Stanley extension 6087).

A Milligen 9050 Fmoc peptide synthesizer has been our workhorse for peptide synthesis, and we have recently acquired a second synthesizer (Perseptive 9050 Plus) through a generous donation from the du Pont Nemours Company in Wilmington, DE and an ABI 331 A recuperated from Geisinger Weiss Research Center. A standard synthesis on either of these machines delivers between 50-80 mg of peptide, at a cost of $20 per residue (0.1 mmol scale), or $30 per residue (0.2 mmol scale). Custom syntheses are also possible (e.g., phosphopeptides, biotinylated peptides), with the additional cost being determined by the reagents used.

Instructions/Charges

Charges: $30 per “standard” amino acid for a full-scale synthesis (~ 0.2 mmol yield) or $20 per standard amino acid residue for a half-scale synthesis (~ 0.1 mmol yield), which covers the cost of the reagents. Your peptide will be prepared as a lyophilized powder cleaved from the resin in the presence of appropriate side group preservatives, extensively washed and extracted following cleavage with various solvents to remove the majority of the impurities (preservatives and byproducts of the cleavage procedure. Extra charges apply for sequence or molecular weight verification (see above). For pricing on any peptides with non-standard amino acids (phosphopeptides, amino acid analogs, etc.) or other specialized preparation procedures which may be necessary for your particular research purpose, please contact Anne Stanley at (717) 531-6087 or by email at astanley@psu.edu.
** ID/Number: This should be a unique alphanumeric label (no more than eight characters long) beginning with your initials or name and ending with letters or numbers which enable you to identify your peptide (e.g., CJL_TOR2). If left blank, we will assign this code.
*** Sequence: Write the requested sequence using traditional three letter or one letter codes. If you need a peptide that is “blocked” with an amide on the carboxyl terminus, end your sequence with an “-NH₂”. Indicate custom amino acids (such as D-amino acids or xenobiotics) with an abbreviation (e.g. X, Abu, XX1, d-Ala). Include information on custom amino acids desired in the "Additional comments..." box as well.

To place an order either download the Peptide Order Form (PDF file), or complete the on-line form below. This on-line form can be used to directly submit up to four Peptide synthesis orders to the PSU College of Medicine Macromolecular Core Facility. To order more than four peptides, please fill out and submit this form again.

***For orders for more than four peptides, do NOT use the "Return to the Form" button at the bottom of the Form Confirmation Page you will see after submitting the order form below; instead, please use your WEB Browser's "Back" arrow to return to the order form below for additional peptide orders.***

Please provide the following contact information

Name

Lab/Dept.

Work Phone

E-mail

Fill in address information below if ordering from outside the Penn State College of Medicine Hershey Campus

Organization

Street address

Address (cont.)

City

State/Province

Zip/Postal code

Peptide 1: Enter an eight-character ID (e.g., CJLTOR23) for the 1st peptide in the space provided below.

Enter the sequence of the 1st peptide you would like to order in the space provided below.

NH2--COOH

Check the appropriate button below for the SCALE OF SYNTHESIS desired for this peptide:

Full-scale synthesis (0.2 mmol, $30 per amino acid)

Half-scale synthesis (0.1 mmol, $20 per amino acid)
After synthesis, do you wish to have this peptide:

Verified by sequencing ($15/residue)
Verified (MW) by MALDI-TOF ($15/peptide)

Additional comments or instructions for Peptide 1

Peptide 2: Enter an eight-character ID (e.g., CJLTOR24) for the 2nd peptide in the space provided below.

Enter the sequence of the 2nd peptide you would like to order in the space provided below.

NH2--COOH

Check the appropriate button below for the SCALE OF SYNTHESIS desired for this peptide:

Full-scale synthesis (0.2 mmol, $30 per amino acid)

Half-scale synthesis (0.1 mmol, $20 per amino acid)

After synthesis, do you wish to have this peptide:

Verified by sequencing ($15/residue)
Verified (MW) by MALDI-TOF ($15/peptide)

Additional comments or instructions for Peptide 2

Peptide 3: Enter an eight-character ID (e.g., CJLTOR25) for the 3rd peptide in the space provided below.

Enter the sequence of the 3rd peptide you would like to order in the space provided below.

NH2--COOH

Check the appropriate button below for the SCALE OF SYNTHESIS desired for this peptide:

Full-scale synthesis (0.2 mmol, $30 per amino acid)

Half-scale synthesis (0.1 mmol, $20 per amino acid)

After synthesis, do you wish to have this peptide:

Verified by sequencing ($15/residue)
Verified (MW) by MALDI-TOF ($15/peptide)

Additional comments or instructions for Peptide 3

Peptide 4: Enter an eight-character ID (e.g., CJLTOR26) for the 4th peptide in the space provided below.

Enter the sequence of the 4th peptide you would like to order in the space below:

NH2--COOH

Check the appropriate button below for the SCALE OF SYNTHESIS desired for this peptide:

Full-scale synthesis (0.2 mmol, $30 per amino acid)

Half-scale synthesis (0.1 mmol, $20 per amino acid)

After synthesis, do you wish to have this peptide:

Verified by sequencing ($15/residue)
Verified (MW) by MALDI-TOF ($15/peptide)

Additional comments or instructions for Peptide 4

Questions? Comments? Contact Anne Stanley, Lab Manager (717) 531-6087, or astanley@psu.edu

Page maintained by Bruce A. Stanley
Last modified February 21, 2008 10:52 PM This page has been viewed Hit Counter times since July 2007

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