Macromolecular
Synthesis
Core
Oligo Synthesis
One
Polygen
synthesizer is used to
generate oligonucleotides. The cost is 33¢ per base,
with no set-up charge. Many users choose to have their oligonucleotides purified, which is done with Nensorb
columns ($15 per purification). Custom syntheses such
as modified bases and phosphorothioate chemistries are
also available, as are larger-scale syntheses.
After submitting an order, you
should see a Confirmation Page . If you DO NOT, then
email your order to
astanley@psu.edu.
Even if you see the Confirmation
Page, if you do
not get an email confirming your order within one day,
check directly with the lab. (Anne Stanley or
Suja Maddukuri,
extension 6087)
Instructions/Charges
- Charges: No set-up charges
$0.33 per nucleotide (0.05 µM scale
synthesis)
$0.66 per nucleotide (0.2 µM scale
synthesis)
$1.00 per nucleotide (1.0µM scale
synthesis)
Nensorb purification -
$15.00 per oligo (0.05 µM, 0.2 µM scale synthesis);
$30.00 per oligo (1.0µM scale
synthesis).
For pricing on any oligos with non-standard
nucleotides (e.g., phosphorothioate oligos), or any other
specialized preparation procedures which may be necessary for
your particular research purpose, please contact Anne Stanley
at (717) 531-6087 or by email at astanley@psu.edu.
-
** ID/Number: This
should be a unique alphanumeric label (no more than six
characters long) beginning with your initials or part of
your name and ending with numbers which enable you to
identify your oligo (e.g., STAN35). If left blank, we
will assign this code.
-
*** Sequence:
Type the requested sequence from the 5' end in groups of
3 capital letters. For EQUAL proportions of mixed bases
at any position, use the IUPAC standard codes, i.e., A/C
= M; A/G = R; A/T = W; C/G = S; C/T = Y; G/T = K; A/C/G
= V; A/C/T = H; A/G/T = D; C/G/T = B; A/C/T/G = N. For
UNEQUAL proportions of mixed bases at any position
("dirty" oligos), please write instructions in the
appropriate box or contact the lab directly.
-
After synthesis, oligos may be picked up from the
top shelf of the freezer in room C1732, with the data
sheets attached to the side of the freezer. An
APPROXIMATE yield of micrograms of oligo will be
included on the data sheet, but because this is
calculated based on the A260 while the oligo is still in
methanol before lyophilization, it is only approximate.
You MUST requantitate the yield and concentration of
each oligo after you have resuspended it in water or TE
buffer in order to have an accurate measure of
yield/concentration.
To place an order either download the
download the
Oligo Order Form
(PDF file), or complete the on-line form below. This
on-line form can be used to directly submit up to four Oligonucleotide synthesis
orders to the PSU College of Medicine Macromolecular Core Facility. To order more than four oligos, please fill out and submit this form again.
***For
orders for more than four oligos, do NOT use the "Return to the Form" button at
the bottom of the Form Confirmation Page you will see after submitting the order
form below; instead, please use your WEB Browser's "Back" arrow to return to the
order form below for additional oligo orders.***
Please provide the following contact information
Questions? Comments? Contact Anne Stanley, Lab Manager (717) 531-6087, or
astanley@psu.edu |
